An assessment of histone-modification antibody quality
Susan Strome, Mitzi I. Kuroda, Andrey A. Gorchakov, Andreas Rechtsteiner, Peter V. Kharchenko, Daniel S. Day, Julie Ahringer, Artyom A. Alekseyenko, Ming-Sin Cheung, Ying Luu, GU Ting-ting, Samantha Kuan, Nicole C. Riddle, Thea A. Egelhofer, Kyungjoon Lee, Queminh Ngo, Marc D. Perry, Gary H. Karpen, Peter J. Park, Sarah Gadel, Gregory Shanower, Yuri B. Schwartz, Bing Ren, Isabel Latorre, Daniela Linder-Basso, Sarah C. R. Elgin, Paulina Kolasinska-Zwierz, Anne Vielle, Vincenzo Pirrotta, Aki Minoda, Sarit Klugman, R David Hawkins
Cited by 0Open Access
Abstract
We report testing of the specificity and utility of over 200 antibodies raised against 57 different histone modifications, in Drosophila melanogaster, Caenorhabditis elegans and human cells. While most antibodies performed well, over 25% failed specificity tests by dot blot or western blot. Among specific antibodies, over 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use and provide a website for posting new test results.
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