Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Andrea Cossarizza(University of Modena and Reggio Emilia), Hyun‐Dong Chang(German Rheumatism Research Centre), Andreas Radbruch(German Rheumatism Research Centre), Sergio Abrignani(University of Milan), Richard Addo(German Rheumatism Research Centre), Mübeccel Akdiş(University of Zurich), Immanuel Andrä(Technical University of Munich), Francesco Andreata(Vita-Salute San Raffaele University), Francesco Annunziato(University of Florence), Eduardo Arranz(Universidad de Valladolid), Petra Bächer(Christian-Albrechts-Universität zu Kiel), Sudipto Bari(Duke-NUS Medical School), Vincenzo Barnaba(Italian Institute of Technology), Joana Barros‐Martins(Medizinische Hochschule Hannover), Dirk Baumjohann(University of Bonn), Cristian G. Beccaría(Vita-Salute San Raffaele University), David Bernardo(Universidad de Valladolid), Dominic A. Boardman(University of British Columbia), Jessica G Borger(Monash University), Chotima Böttcher(Humboldt-Universität zu Berlin), Leonie Brockmann(Columbia University), Marie E. Burns(German Rheumatism Research Centre), Dirk H. Busch(German Center for Infection Research), Garth Cameron(Australian Research Council), Ilenia Cammarata(Sapienza University of Rome), Antonino Cassotta(Università della Svizzera italiana), Yinshui Chang(University of Bonn), Fernando G. Chirdo(Consejo Nacional de Investigaciones Científicas y Técnicas), Eleni Christakou(King's College London), Luka Čičin‐Šain(Helmholtz Centre for Infection Research), Laura Cook(University of British Columbia), Alexandra J. Corbett(The University of Melbourne), Rebecca Cornelis(German Rheumatism Research Centre), Lorenzo Cosmi(University of Florence), Martin S. Davey(Discovery Institute), Sara De Biasi(University of Modena and Reggio Emilia), Gabriele De Simone(IRCCS Humanitas Research Hospital), Genny Del Zotto(Istituto Giannina Gaslini), Michael Delacher(Johannes Gutenberg University Mainz), Francesca Di Rosa(The Francis Crick Institute), James P. Di Santo(Inserm), Andreas Diefenbach(German Rheumatism Research Centre), Jun Dong(Leibniz Association), Thomas Dörner(German Rheumatism Research Centre), Regine J. Dress(Universität Hamburg), Charles‐Antoine Dutertre(Inserm), Sidonia B. G. Eckle(The University of Melbourne), Pascale Eede(Humboldt-Universität zu Berlin), Maximilien Evrard(Agency for Science, Technology and Research), Christine S. Falk(Medizinische Hochschule Hannover), Markus Feuerer(University Hospital Regensburg), Simon Fillatreau(Délégation Paris 5), Aida Fiz‐López(Universidad de Valladolid), Marie Follo(University of Freiburg), Gemma A. Foulds(Nottingham Trent University), Julia Fröbel(Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)), Nicola Gagliani(Universität Hamburg), Giovanni Galletti(IRCCS Humanitas Research Hospital), Anastasia Gangaev(The Netherlands Cancer Institute), Natalio Garbi(University of Bonn), José Antonio Garrote(Universidad de Valladolid), Jens Geginat(University of Milan), Nicholas A. Gherardin(Australian Research Council), Lara Gibellini(University of Modena and Reggio Emilia), Florent Ginhoux(Agency for Science, Technology and Research), Dale I. Godfrey(Australian Research Council), Paola Gruarin(Istituto Nazionale Genetica Molecolare), Claudia Haftmann(University of Zurich), Leo Hansmann(German Cancer Society), Christopher M. Harpur(Hudson Institute of Medical Research), Adrian Hayday(King's College London), Guido Heine(University Hospital Schleswig-Holstein), Daniela Hernandez(Humboldt-Universität zu Berlin), Martin Herrmann(Friedrich-Alexander-Universität Erlangen-Nürnberg), Oliver Hoelsken(German Rheumatism Research Centre), Qing Huang(University of British Columbia), Samuel Huber(Universität Hamburg), Johanna E. Huber(Urologische Klinik München), Jochen Huehn(Helmholtz Centre for Infection Research), Michael Hundemer(Heidelberg University), William Ying Khee Hwang(Singapore General Hospital), Matteo Iannacone(Vita-Salute San Raffaele University), Sabine Ivison(University of British Columbia), Hans‐Martin Jäck(Friedrich-Alexander-Universität Erlangen-Nürnberg), Péter K. Jani(German Rheumatism Research Centre), Baerbel Keller(University of Freiburg), Nina Kessler(University of Bonn), Steven L. C. Ketelaars(The Netherlands Cancer Institute), Laura Knop(Otto-von-Guericke-Universität Magdeburg), Jasmin Knopf(Friedrich-Alexander-Universität Erlangen-Nürnberg), Hui‐Fern Koay(Australian Research Council), Katja Kobow(Universitätsklinikum Erlangen), Katharina Kriegsmann(Heidelberg University), Hendy Kristyanto(Leiden University Medical Center), Andreas Krueger(Goethe University Frankfurt), J.F. Kuehne(Medizinische Hochschule Hannover), Heike Kunze‐Schumacher(Goethe University Frankfurt), Pia Kvistborg(The Netherlands Cancer Institute), Immanuel Kwok(Agency for Science, Technology and Research), Daniela Latorre(ETH Zurich), Daniel Lenz(German Rheumatism Research Centre), Megan K. Levings(University of British Columbia), Andreia C. Lino(German Rheumatism Research Centre), Francesco Liotta(University of Florence), Heather M. Long(University of Birmingham), Enrico Lugli(IRCCS Humanitas Research Hospital), Katherine N. MacDonald(University of British Columbia), Laura Maggi(University of Florence), Mala K. Maini(University College London), Florian Mair(Fred Hutch Cancer Center), Calin Manta(Heidelberg University), Rudolf A. Manz(Institute for Integrative and Experimental Genomics), Mir‐Farzin Mashreghi(German Rheumatism Research Centre), Alessio Mazzoni(University of Florence), James McCluskey(The University of Melbourne), Henrik E. Mei(German Rheumatism Research Centre), Fritz Melchers(German Rheumatism Research Centre), Susanne Melzer(Leipzig University), Dirk Mielenz(Friedrich-Alexander-Universität Erlangen-Nürnberg), Leticia Monin(The Francis Crick Institute), Lorenzo Moretta(Bambino Gesù Children's Hospital), Gabriele Multhoff(TUM Klinikum), Luis E. Muñoz(Friedrich-Alexander-Universität Erlangen-Nürnberg), Miguel Muñoz‐Ruiz(The Francis Crick Institute), Franziska Muscate(Universität Hamburg), Ambra Natalini(Institute of Molecular Biology and Pathology), Katrin Neumann(Universität Hamburg), Lai Guan Ng(Agency for Science, Technology and Research), Antonia Niedobitek(German Rheumatism Research Centre), Jana Niemz(Helmholtz Centre for Infection Research), Larissa N. Almeida(Institute for Integrative and Experimental Genomics), Samuele Notarbartolo(Istituto Nazionale Genetica Molecolare), Lennard Ostendorf(Humboldt-Universität zu Berlin), Laura J. Pallett(University College London), Amit A. Patel(Inserm), Gülce Itır Perçin(Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)), Giovanna Peruzzi(Italian Institute of Technology), Marcello Pinti(University of Modena and Reggio Emilia), A. Graham Pockley(Nottingham Trent University), Katharina Pracht(Friedrich-Alexander-Universität Erlangen-Nürnberg), Immo Prinz(Universität Hamburg), Irma Pujol‐Autonell(King's College London), Nadia Pulvirenti(Istituto Nazionale Genetica Molecolare), Linda Quatrini(Bambino Gesù Children's Hospital), Kylie M. Quinn(Monash University), Helena Radbruch(Humboldt-Universität zu Berlin), Hefin Rhys(The Francis Crick Institute), Maria B. Rodrigo(University of Bonn), Chiara Romagnani(Humboldt-Universität zu Berlin), Carina Saggau(Christian-Albrechts-Universität zu Kiel), Shimon Sakaguchi(Ube Frontier University), Federica Sallusto(ETH Zurich), Lieke Sanderink(University Hospital Regensburg), Inga Sandrock(Medizinische Hochschule Hannover), Christine Schauer(Friedrich-Alexander-Universität Erlangen-Nürnberg), Alexander Scheffold(Christian-Albrechts-Universität zu Kiel), Hans Ulrich Scherer(Leiden University Medical Center), Matthias Schiemann(Technical University of Munich), Frank A. Schildberg(University Hospital Bonn), Kilian Schober(Universitätsklinikum Erlangen), Janina Schoen(Friedrich-Alexander-Universität Erlangen-Nürnberg), Wolfgang Schuh(Friedrich-Alexander-Universität Erlangen-Nürnberg), Thomas Schüler(Otto-von-Guericke-Universität Magdeburg), Axel Schulz(German Rheumatism Research Centre), Sebastian Schulz(Friedrich-Alexander-Universität Erlangen-Nürnberg), Julia Schulze(German Rheumatism Research Centre), Sonia Simonetti(Institute of Molecular Biology and Pathology), Jeeshan Singh(Friedrich-Alexander-Universität Erlangen-Nürnberg), Katarzyna Sitnik(Helmholtz Centre for Infection Research), Regina Stark(Sanquin), Sarah C. Starossom(Humboldt-Universität zu Berlin), Christina Stehle(Humboldt-Universität zu Berlin), Franziska Szelinski(German Rheumatism Research Centre), Leonard Tan(Agency for Science, Technology and Research), Attila Tárnok(Fraunhofer Institute for Cell Therapy and Immunology), Julia Tornack(German Rheumatism Research Centre), Timothy Tree(King's College London), Jasper J. P. van Beek(IRCCS Humanitas Research Hospital), Willem van de Veen(University of Zurich), Klaas P. J. M. van Gisbergen(Sanquin), Chiara Vasco(Istituto Nazionale Genetica Molecolare), Nikita A. Verheyden(Goethe University Frankfurt), Anouk von Borstel(Discovery Institute), Kirsten A. Ward‐Hartstonge(University of British Columbia), Klaus Warnatz(University of Freiburg), Claudia Waskow(Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)), Annika Wiedemann(German Rheumatism Research Centre), Anneke Wilharm(Medizinische Hochschule Hannover), James B. Wing(Ube Frontier University), Oliver F. Wirz(Stanford University), Jens Wittner(Friedrich-Alexander-Universität Erlangen-Nürnberg), Jennie H. M. Yang(King's College London), Juhao Yang(King's College London)
Cited by 471Open Access
Abstract
The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.
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