NANOS2 is a sequence-specific mRNA-binding protein that promotes transcript degradation in spermatogonial stem cells
Azzurra Codino(MRC Centre for Regenerative Medicine), Tomasz W. Turowski(Wellcome Centre for Cell Biology), Louie N. van de Lagemaat(Queen Mary University of London), Ivayla Ivanova(MRC Centre for Regenerative Medicine), Andrea Tavosanis(Queen Mary University of London), Christian Much(MRC Centre for Regenerative Medicine), Tania Auchynnikava(Wellcome Centre for Cell Biology), Lina Vasiliauskaitė(MRC Centre for Regenerative Medicine), Marcos Morgan(MRC Centre for Regenerative Medicine), Juri Rappsilber(Wellcome Centre for Cell Biology), Robin C. Allshire(Wellcome Centre for Cell Biology), Kamil R. Kranc(Queen Mary University of London), David Tollervey(Wellcome Centre for Cell Biology), Dónal O’Carroll(MRC Centre for Regenerative Medicine)
Cited by 19Open Access
Abstract
mouse allele and applied the highly stringent cross-linking and analysis of cDNAs to define NANOS2 RNA occupancy in SSC lines. NANOS2 recognizes the AUKAAWU consensus motif, mostly found in the 3' untranslated region of defined messenger RNAs (mRNAs). We find that NANOS2 is a regulator of key signaling and metabolic pathways whose dosage or activity are known to be critical for SSC maintenance. NANOS2 interacts with components of CCR4-NOT deadenylase complex in SSC lines, and consequently, NANOS2 binding reduces the half-lives of target transcripts. In summary, NANOS2 contributes to SSC maintenance through the regulation of target mRNA stability and key self-renewal pathways.
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