Intrinsic signal amplification by type III CRISPR-Cas systems provides a sequence-specific SARS-CoV-2 diagnostic
Andrew Santiago‐Frangos(Montana State University), L Hall(Montana State University), Anna Nemudraia(Montana State University), Artem Nemudryi(Montana State University), Pushya Krishna(Montana State University), Tanner Wiegand(Montana State University), Royce A. Wilkinson(Montana State University), Deann T. Snyder(Montana State University), Jodi F. Hedges(Montana State University), Calvin Cicha(Montana State University), Helen Lee(Montana State University), Ava B. Graham(Montana State University), Mark A. Jutila(Montana State University), Matthew P. Taylor(Montana State University), Blake Wiedenheft(Montana State University)
Cited by 87Open Access
Abstract
copies/μL for ten guides. To decrease the limit of detection to levels that are clinically relevant, we developed a two-pot reaction consisting of RT-LAMP followed by T7-transcription and type III CRISPR-based detection. The two-pot reaction has a sensitivity of 200 copies/μL and is completed using patient samples in less than 30 min.
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