Protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver
Liliana Sokol(VIB-KU Leuven Center for Cancer Biology), Vincent Geldhof(VIB-KU Leuven Center for Cancer Biology), Melissa García‐Caballero(VIB-KU Leuven Center for Cancer Biology), Nadine V. Conchinha(VIB-KU Leuven Center for Cancer Biology), Sébastien J. Dumas(VIB-KU Leuven Center for Cancer Biology), Elda Meta(VIB-KU Leuven Center for Cancer Biology), Laure-Anne Teuwen(University of Antwerp), Koen Veys(VIB-KU Leuven Center for Cancer Biology), Rongyuan Chen(Sun Yat-sen University), Lucas Treps(VIB-KU Leuven Center for Cancer Biology), Mila Borri(VIB-KU Leuven Center for Cancer Biology), Pauline de Zeeuw(VIB-KU Leuven Center for Cancer Biology), Kim D. Falkenberg(VIB-KU Leuven Center for Cancer Biology), Charlotte Dubois(VIB-KU Leuven Center for Cancer Biology), Magdalena Parys(VIB-KU Leuven Center for Cancer Biology), Laura de Rooij(VIB-KU Leuven Center for Cancer Biology), Jermaine Goveia(VIB-KU Leuven Center for Cancer Biology), Kateřina Rohlenová(VIB-KU Leuven Center for Cancer Biology), Luc Schoonjans(Sun Yat-sen University), Mieke Dewerchin(VIB-KU Leuven Center for Cancer Biology), Guy Eelen(VIB-KU Leuven Center for Cancer Biology), Xuri Li(Sun Yat-sen University), Joanna Kalucka(Aarhus University), Peter Carmeliet(Sun Yat-sen University)
Cited by 27Open Access
Abstract
Endothelial cells (ECs) from the small intestine, colon, liver, and heart have distinct phenotypes and functional adaptations that are dependent on their physiological environment. Gut ECs adapt to low oxygen, heart ECs to contractile forces, and liver ECs to low flow rates. Isolating high-purity ECs in sufficient quantities is crucial to study their functions. Here, we describe protocols combining magnetic and fluorescent activated cell sorting for rapid and reproducible EC purification from four adult murine tissues. For complete details on the use and execution of these protocols, please refer to Kalucka et al. (2020).
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