<i>De Novo</i> Discovery of High-Affinity Peptide Binders for the SARS-CoV-2 Spike Protein
Sebastian Pomplun(Massachusetts Institute of Technology), Muhammad Jbara(Massachusetts Institute of Technology), Anthony J. Quartararo(Massachusetts Institute of Technology), Genwei Zhang(Massachusetts Institute of Technology), Joseph S. Brown(Massachusetts Institute of Technology), Yen‐Chun Lee(Massachusetts Institute of Technology), Xiyun Ye(Massachusetts Institute of Technology), Stephanie Hanna(Massachusetts Institute of Technology), Bradley L. Pentelute(Broad Institute)
Cited by 92Open Access
Abstract
= 80-970 nM) for RBD and selectivity over human serum proteins. Nanomolar RBD concentrations in a biological matrix could be detected using the biotinylated lead peptide in ELISA format. These peptides do not compete for ACE2 binding, and their site of interaction on the SARS-CoV-2-spike-RBD might be unrelated to the ACE2 binding site, making them potential orthogonal reagents for sandwich immunoassays. These findings serve as a starting point for the development of SARS-CoV-2 diagnostics or conjugates for virus-directed delivery of therapeutics.
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