Evaluation of a SARS-CoV-2 Surrogate Virus Neutralization Test for Detection of Antibody in Human, Canine, Cat, and Hamster Sera
Ranawaka A. P. M. Perera(University of Hong Kong), Ronald LW Ko(University of Hong Kong), Owen TY Tsang(Hospital Authority), David S.C. Hui(Chinese University of Hong Kong), Mike Yat Wah Kwan(Hospital Authority), Christopher J. Brackman(Agriculture Fisheries and Conservation Department), Esther M. W. To(Agriculture Fisheries and Conservation Department), Hui‐Ling Yen(University of Hong Kong), Kathy Leung(Laboratory of Data Discovery for Health), Samuel M. S. Cheng(University of Hong Kong), Kin Ho Chan(University of Hong Kong), Kaimin Chan(University of Hong Kong), Ka-Chi Li(University of Hong Kong), Linda J. Saif(The Ohio State University), Vanessa R. Barrs(City University of Hong Kong), Joseph T. Wu(Laboratory of Data Discovery for Health), Thomas H. C. Sit(Agriculture Fisheries and Conservation Department), Leo L. M. Poon(HKU-Pasteur Research Pole), Malik Peiris(HKU-Pasteur Research Pole)
Cited by 136Open Access
Abstract
Surrogate neutralization assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that can be done without biosafety level 3 containment and in multiple species are desirable. We evaluate a recently developed surrogate virus neutralization test (sVNT) in comparison to 90% plaque reduction neutralization tests (PRNT 90 ) in human, canine, cat, and hamster sera. With PRNT 90 as the reference, sVNT had sensitivity of 98.9% and specificity of 98.8%. Using a panel of immune sera corresponding to other coronaviruses, we confirm the lack of cross-reactivity to other coronaviruses in SARS-CoV-2 sVNT and PRNT 90 , except for cross-reactivity to SARS-CoV-1 in sVNT.
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