The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020

The dMIQE Group(National Physical Laboratory), Alexandra S. Whale(National Physical Laboratory), Ward De Spiegelaere(Ghent University Hospital), Wim Trypsteen(Holy Spirit University of Kaslik), Afif M. Abdel Nour(Holy Spirit University of Kaslik), Young‐Kyung Bae(Korea Research Institute of Standards and Science), Vladimı́r Beneš(National Measurement Institute), Daniel Burke(National Measurement Institute), Megan H. Cleveland(National Institute of Standards and Technology), Philippe Corbisier(National Physical Laboratory), Alison S. Devonshire(National Physical Laboratory), Lianhua Dong(National Institute of Metrology), Daniela Drandi(National Physical Laboratory), Carole A. Foy(National Physical Laboratory), Jeremy A. Garson(National Institute of Standards and Technology), Hua‐Jun He(National Institute of Standards and Technology), Jan Hellemans(Tataa Biocenter (Sweden)), Mikael Kubista(European Commission), Antoon Lievens(Harvard University), Mike Makrigiorgos(Harvard University), Mojca Milavec(National Institute of Biology), Reinhold Mueller(Manchester Academic Health Science Centre), Tania Nolan(National Physical Laboratory), Denise M. O’Sullivan(National Physical Laboratory), Michael W. Pfaffl(University of Potsdam), Stefan Rödiger(National Institute of Standards and Technology), Erica L. Romsos(National Institute of Standards and Technology), Gregory L. Shipley(Inserm), Valérie Taly(Inserm), Andreas Untergasser(University of Utah), Carl T. Wittwer(University of Utah), Stephen A. Bustin(Ghent University Hospital), Jo Vandesompele(National Physical Laboratory), Jim F. Huggett(National Physical Laboratory)
Clinical Chemistry
June 15, 2020
10.1093/clinchem/hvaa125
Cited by 497Open Access
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Abstract

Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology.

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