Multiomic analysis of a dried single-drop plasma sample using an integrated mass spectrometry approach
Weina Gao(Harbin Institute of Technology), Qiaoyun Zhang(Harbin Institute of Technology), Yiran Su(Southern University of Science and Technology), Peiwu Huang(Southern University of Science and Technology), Xue Lu(Southern University of Science and Technology), Qinyue Gong(Southern University of Science and Technology), Wendong Chen(Southern University of Science and Technology), Ruilian Xu(Southern University of Science and Technology), Ruijun Tian(Southern University of Science and Technology)
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Abstract
An easy-to-use and fast approach was developed for integrated proteomic and metabolic profiling in a dried single-drop plasma sample. Plasma collection, room temperature storage, and sample preparation for both proteins and metabolites were seamlessly integrated in one spintip device. MS-based multiomic profiling using the same nano LC-MS system identified more than 150 proteins and 160 metabolites from the 1 μL plasma sample in 6 hours. Further combination with micro-flow LC and targeted MS made it a promising approach for the fast profiling of molecular biomarkers with high sensitivity and accuracy.
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