Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize
Yuanyuan Jiang(China Agricultural University), Yiping Chai(China Agricultural University), Minhui Lu(China Agricultural University), Xiuli Han(China Agricultural University), Qiupeng Lin(Chinese Academy of Sciences), Yingxin Zhang(China Agricultural University), Qiang Zhang(China Agricultural University), Yun Zhou(Henan University), Xuechen Wang(China Agricultural University), Caixia Gao(Chinese Academy of Sciences), Qijun Chen(China Agricultural University)
Cited by 34Open Access
Abstract
Abstract A novel and universal CRISPR/Cas-derived precision genome-editing technology named prime editing was developed. However, low efficiency of prime editing was shown in transgenic rice lines. We reasoned that enhancing pegRNA expression would improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We constructed a prime-editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2 . Compared with previous reports in rice, we achieved much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors.
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