<i>Pyrococcus furiosus</i> Argonaute-mediated nucleic acid detection
Ruyi He(Center for Innovation), Longyu Wang(Center for Innovation), Fei Wang(Center for Innovation), Wenqiang Li(Center for Innovation), Yang Liu(Center for Innovation), Aitao Li(Center for Innovation), Yuan Wang(Hubei University of Arts and Science), Wuxiang Mao(Center for Innovation), Chao Zhai(Center for Innovation), Lixin Ma(Center for Innovation)
Cited by 159
Abstract
We demonstrate that short single-stranded DNA generated by Pyrococcus furiosus Argonaute (PfAgo) can initiate a second round of cleavage. Based on this principle, we established a molecular diagnostic method, termed PfAgo-mediated Nucleic acid Detection (PAND). This method could detect DNA at attomolar sensitivities, distinguish single-nucleotide mutants and accomplish multiplexed detection.
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