CRISPR-TSKO: A Technique for Efficient Mutagenesis in Specific Cell Types, Tissues, or Organs in Arabidopsis
Ward Decaestecker(Ghent University), Rafael Andrade Buono(Ghent University), Marie L. Pfeiffer(Ghent University), Nick Vangheluwe(Ghent University), Joris Jourquin(Ghent University), Mansour Karimi(Ghent University), Gert Van Isterdael(Ghent University), Tom Beeckman(Ghent University), Moritz K. Nowack(Ghent University), Thomas B. Jacobs(Ghent University)
Cited by 260Open Access
Abstract
), CRISPR-TSKO mutations in essential genes caused well-defined, localized phenotypes in the root cap, stomatal lineage, or entire lateral roots. The modular cloning system developed in this study allows for the efficient selection, identification, and functional analysis of mutant lines directly in the first transgenic generation. The efficacy of CRISPR-TSKO opens avenues for discovering and analyzing gene functions in the spatial and temporal contexts of plant life while avoiding the pleiotropic effects of system-wide losses of gene function.
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