A lineage-resolved molecular atlas of <i>C. elegans</i> embryogenesis at single-cell resolution
Jonathan S. Packer(University of Washington), Qin Zhu(University of Pennsylvania), Chau Huynh(University of Washington), Priya Sivaramakrishnan(University of Pennsylvania), Elicia Preston(University of Pennsylvania), Hannah Dueck(University of Pennsylvania), Derek Stefanik(University of Pennsylvania), Kai Tan(Children's Hospital of Philadelphia), Cole Trapnell(University of Washington), Junhyong Kim(University of Pennsylvania), R Waterston(University of Washington), John I. Murray(University of Pennsylvania)
Cited by 622Open Access
Abstract
Identifying terminal nematode cells Single-cell RNA sequencing provides the power to identify the developmental trajectory of an organism. However, identifying the temporal lineage of cell development can be difficult without large-scale analyses. Packer et al. sequenced more than 80,000 cells from embryos of the roundworm Caenorhabditis elegans to determine the expression of genes directing the development of terminal cell types. Because all somatic cells in a C. elegans individual have been mapped, the authors are able to connect gene expression with cell lineages over time during development, noting stark transitions in some cases. Science , this issue p. eaax1971