RNA-guided DNA insertion with CRISPR-associated transposases
Jonathan Strecker(Broad Institute), Alim Ladha(Broad Institute), Zachary Gardner(Broad Institute), Jonathan L. Schmid‐Burgk(Broad Institute), Kira S. Makarova(National Institutes of Health), Eugene V. Koonin(National Institutes of Health), Feng Zhang(Broad Institute)
Cited by 709Open Access
Abstract
Beyond adaptive immunity Prokaryotic CRISPR-Cas systems defend bacterial cells from phage and plasmid infection. Strecker et al. characterized a CRISPR-Cas system that functions beyond adaptive immunity (see the Perspective by Hou and Zhang). Type V-K CRISPR-Cas from cyanobacteria was associated with a Tn7-like transposon and a natural nuclease–deficient effector Cas12k. Cas12k directed the insertion of Tn7-like transposons into target sites via RNA-guided Tn7 transposition. This system was reprogrammed to efficiently and specifically insert DNA both in vitro and into the Escherichia coli genome. Science , this issue p. 48 ; see also p. 25
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