Manufacture of Chimeric Antigen Receptor T Cells from Mobilized Cyropreserved Peripheral Blood Stem Cell Units Depends on Monocyte Depletion
Annette Künkele(Berlin Institute of Health at Charité - Universitätsmedizin Berlin), Christopher Brown, Adam Beebe, Stephanie Mgebroff, Adam Johnson(Seattle Children's Hospital), Agne Taraseviciute(University of Washington), Lisa S. Rolczynski(Seattle Children's Hospital), Cindy A. Chang(Seattle Children's Hospital), Olivia Finney(Seattle Children's Hospital), Julie R. Park(University of Washington), Michael C. Jensen(Fred Hutch Cancer Center)
Cited by 36Open Access
Abstract
CD4 and CD8 effector cells derived from cryopreserved units displayed antineuroblastoma lytic potency and cytokine secretion comparable to those derived from a healthy donor and mediated in vivo antitumor regression in NSG mice. We conclude that cryopreserved PBSCs procured via standard methods during early treatment can serve as an alternative starting source for CAR-T cell manufacturing, extending the options for heavily treated patients.
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