Consensus guidelines for the use and interpretation of angiogenesis assays
Patrycja Nowak‐Sliwinska(University of Geneva), Kari Alitalo(University of Helsinki), Elizabeth Allen(VIB-KU Leuven Center for Cancer Biology), Andrey Anisimov(University of Helsinki), Alfred C. Aplin(University of Washington), Robert Auerbach(University of Wisconsin–Madison), Hellmut G. Augustin(German Cancer Research Center), David O. Bates(University of Nottingham), Judy R. van Beijnum(Cancer Center Amsterdam), R. Hugh F. Bender(University of California, Irvine), Gabriele Bergers(VIB-KU Leuven Center for Cancer Biology), Andréas Bikfalvi(Inserm), Joyce Bischoff(Boston Children's Hospital), Barbara C. Böck(German Cancer Research Center), Peter C. Brooks(Maine Medical Center), Federico Bussolino(Candiolo Cancer Institute), Bertan Cakir(Boston Children's Hospital), Peter Carmeliet(VIB-KU Leuven Center for Cancer Biology), Daniel Castranova(National Institutes of Health), Anca Maria Cîmpean(Victor Babeș University of Medicine and Pharmacy Timișoara), Ondine Cleaver(The University of Texas Southwestern Medical Center), George Coukos(Ludwig Cancer Research), George E. Davis(University of Missouri), Michele De Palma(École Polytechnique Fédérale de Lausanne), Anna Dimberg(Uppsala University), Ruud P.M. Dings(University of Arkansas for Medical Sciences), Valentin Djonov(University of Bern), Andrew C. Dudley(University of Virginia), Neil Dufton(Imperial College London), Sarah‐Maria Fendt(VIB-KU Leuven Center for Cancer Biology), Napoleone Ferrara(University of California San Diego), Marcus Fruttiger(University College London), Dai Fukumura(Harvard University), Bart Ghesquière(VIB-KU Leuven Center for Cancer Biology), Yan Gong(Boston Children's Hospital), Robert J. Griffin(University of Arkansas for Medical Sciences), Adrian L. Harris(John Radcliffe Hospital), Christopher C.W. Hughes(University of California, Irvine), Nan W. Hultgren(University of California, Irvine), M. Luisa Iruela‐Arispe(University of California, Los Angeles), Melita Irving(Ludwig Cancer Research), Rakesh K. Jain(Harvard University), Raghu Kalluri(The University of Texas MD Anderson Cancer Center), Joanna Kalucka(VIB-KU Leuven Center for Cancer Biology), Robert S. Kerbel(Sunnybrook Health Science Centre), Jan Kitajewski(University of Illinois Chicago), Ingeborg Klaassen(Amsterdam UMC Location University of Amsterdam), Hynda K. Kleinmann(George Washington University), Pieter Koolwijk(Fondation Asile des Aveugles), Elisabeth Kuczynski(Sunnybrook Health Science Centre), Brenda R. Kwak(University of Geneva), Koen M. Marien(HistoGeneX (Belgium)), Juan M. Melero‐Martin(Boston Children's Hospital), Lance L. Munn(Harvard University), Roberto F. Nicosia(University of Washington), Agnès Noël(University of Liège), Jussi Nurro(University of Eastern Finland), Anna-Karin Olsson(Uppsala University), Tatiana V. Petrova(Ludwig Cancer Research), Kristian Pietras(Lund University), Роберто Пили(Indiana University Health), Jeffrey W. Pollard(MRC Centre for Reproductive Health), Mark J. Post(Maastricht University), Paul H.A. Quax, Gabriel A. Rabinovich(Consejo Nacional de Investigaciones Científicas y Técnicas), Marius Raica(Victor Babeș University of Medicine and Pharmacy Timișoara), Anna M. Randi(Imperial College London), Doménico Ribatti(Istituto Tumori Bari), Curzio Rüegg(University of Fribourg), Reinier O. Schlingemann(Amsterdam UMC Location University of Amsterdam), Stefan Schulte‐Merker, Lois E. H. Smith(Boston Children's Hospital), Jonathan W. Song(The Ohio State University), Steven A. Stacker(The University of Melbourne), Jimmy Stalin, Amber N. Stratman(National Institutes of Health), Maureen Van de Velde(University of Liège), Victor W.M. van Hinsbergh(Fondation Asile des Aveugles), Peter Vermeulen(GZA Ziekenhuizen Campus Sint-Augustinus), Johannes Waltenberger(University of Münster), Brant M. Weinstein(National Institutes of Health), Hong Xin(University of California San Diego), Bahar Yetkin-Arik(Amsterdam UMC Location University of Amsterdam), Seppo Ylä‐Herttuala(University of Eastern Finland), Mervin C. Yöder(Indiana University School of Medicine), Arjan W. Griffioen(Cancer Center Amsterdam)
Cited by 593Open Access
Abstract
The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference.
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