Choice of costimulatory domains and of cytokines determines CAR T-cell activity in neuroblastoma
Concetta Quintarelli(Bambino Gesù Children's Hospital), Domenico Orlando(Bambino Gesù Children's Hospital), Iolanda Boffa(Bambino Gesù Children's Hospital), Marika Guercio(Bambino Gesù Children's Hospital), Vinicia Assunta Polito(Bambino Gesù Children's Hospital), Andrea Petretto(Istituto Giannina Gaslini), Chiara Lavarello(Istituto Giannina Gaslini), Matilde Sinibaldi(Bambino Gesù Children's Hospital), Gerrit Weber(Bambino Gesù Children's Hospital), Francesca Del Bufalo(Bambino Gesù Children's Hospital), Ezio Giorda(Bambino Gesù Children's Hospital), Marco Scarsella(Bambino Gesù Children's Hospital), Stefania Petrini(Bambino Gesù Children's Hospital), Daria Pagliara(Bambino Gesù Children's Hospital), Franco Locatelli(University of Pavia), Biagio De Angelis(Bambino Gesù Children's Hospital), Ignazio Caruana(Bambino Gesù Children's Hospital)
Cited by 157Open Access
Abstract
tumor control and 4) T-cell persistence. The fine-tuning of T-cell culture conditions obtained using IL7 and IL15 was found to be synergic with the CAR.GD2 design in increasing the anti-tumor activity of CAR T cells. We also demonstrate that activation of the suicide gene iC9, included in our construct without significantly impairing neither CAR expression nor anti-tumor activity, leads to a prompt induction of apoptosis of GD2.CAR T cells. Altogether, these findings are instrumental in optimizing the function of CAR T-cell products to be employed in the treatment of children with NB.
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