Guidelines for the use of flow cytometry and cell sorting in immunological studies <sup>*</sup>
Andrea Cossarizza(University of Modena and Reggio Emilia), Hyun‐Dong Chang(Leibniz Association), Andreas Radbruch(Leibniz Association), Mübeccel Akdiş, Immanuel Andrä(Technical University of Munich), Francesco Annunziato(University of Florence), Petra Bächer(Charité - Universitätsmedizin Berlin), Vincenzo Barnaba(Istituto Pasteur), Luca Battistini(Fondazione Santa Lucia), Wolfgang Bauer(Medical University of Vienna), Sabine Baumgart(Leibniz Association), Burkhard Becher(University of Zurich), Wolfgang Beisker(Center for Environmental Health), Claudia Berek(Leibniz Association), Alfonso Blanco(University College Dublin), Giovanna Borsellino(Fondazione Santa Lucia), Philip E. Boulais(Albert Einstein College of Medicine), Ryan R. Brinkman(BC Cancer Agency), Martin Büscher(Miltenyi Biotec (Germany)), Dirk H. Busch(German Center for Infection Research), Timothy Bushnell(University of Rochester Medical Center), Xuetao Cao(Second Military Medical University), Andrea Cavani, Pratip K. Chattopadhyay, Qingyu Cheng(Charité - Universitätsmedizin Berlin), Sue Chow(Princess Margaret Cancer Centre), Mario Clerici(University of Milan), Anne Cooke(University of Cambridge), Antonio Cosma(Université Paris-Sud), Lorenzo Cosmi(University of Florence), Ana Cumano(Institut Pasteur), Van Duc Dang(Leibniz Association), Derek Davies(The Francis Crick Institute), Sara De Biasi(University of Modena and Reggio Emilia), Genny Del Zotto(Istituto Giannina Gaslini), Silvia Della Bella(Humanitas University), Paolo Dellabona(Vita-Salute San Raffaele University), Günnur Deniz(Istanbul University), Mark C. Dessing(Sony (United Kingdom)), Andreas Diefenbach(Charité - Universitätsmedizin Berlin), James P. Di Santo(Institut Pasteur), Francesco Dieli(University of Palermo), Andreas Dolf(University of Bonn), Vera S. Donnenberg(University of Pittsburgh), Thomas Dörner(Charité - Universitätsmedizin Berlin), Götz R. A. Ehrhardt(University of Toronto), Elmar Endl(University of Bonn), Pablo Engel(Universitat de Barcelona), Britta Engelhardt(University of Bern), Charlotte Esser(Leibniz Institute of Environmental Medicine), Bart Everts(Leiden University Medical Center), Anita Dreher, Christine S. Falk(Medizinische Hochschule Hannover), Todd A. Fehniger(Washington University in St. Louis), Andrew Filby(Newcastle University), Simon Fillatreau(Délégation Paris 5), Marie Follo(University of Freiburg), Irmgard Förster(University of Bonn), John R. Foster(Owl Biomedical (United States)), Gemma A. Foulds(Nottingham Trent University), Paul S. Frenette(Albert Einstein College of Medicine), David W. Galbraith(University of Arizona), Natalio Garbi(University of Bonn), Maria Dolores García‐Godoy(Josep Carreras Leukaemia Research Institute), Jens Geginat(Istituto Nazionale Genetica Molecolare), Kamran Ghoreschi(University of Tübingen), Lara Gibellini(University of Modena and Reggio Emilia), Christoph Goettlinger, Carl S. Goodyear(University of Glasgow), Andrea Gori(University of Milano-Bicocca), Jane L. Grogan, Mor Gross(Weizmann Institute of Science), Andreas Grützkau(Leibniz Association), Daryl Grummitt(Owl Biomedical (United States)), Jonas Hahn(Friedrich-Alexander-Universität Erlangen-Nürnberg), Quirin Hammer(Leibniz Association), Anja E. Hauser(Leibniz Association), David L. Haviland(Houston Methodist), David W. Hedley(Princess Margaret Cancer Centre), Guadalupe Herrera(Universitat de València), Martin Herrmann(Friedrich-Alexander-Universität Erlangen-Nürnberg), Falk Hiepe(Charité - Universitätsmedizin Berlin), Tristan Holland, Pleun Hombrink(Sanquin), Jessica P. Houston(New Mexico State University), Bimba F. Hoyer(Charité - Universitätsmedizin Berlin), Bo Huang(Chinese Academy of Medical Sciences & Peking Union Medical College), Christopher A. Hunter(University of Pennsylvania), Anna Iannone(University of Modena and Reggio Emilia), Hans‐Martin Jäck(Universitätsklinikum Erlangen), Beatriz Jávega(Universitat de València), Stipan Jonjić(University of Rijeka), Kerstin Juelke(Leibniz Association), Steffen Jung(Weizmann Institute of Science), Toralf Kaiser(Leibniz Association), Tomáš Kalina(Charles University), Baerbel Keller(University of Freiburg), Srijit Khan(University of Toronto), Deborah Kienhöfer(Friedrich-Alexander-Universität Erlangen-Nürnberg), Thomas Kroneis(Medical University of Graz), Désirée Kunkel(Berlin-Brandenburger Centrum für Regenerative Therapien), Christian Kurts(University of Bonn), Pia Kvistborg(The Netherlands Cancer Institute), Joanne Lannigan(University of Virginia), Olivier Lantz(Inserm), Anis Larbi(Agency for Science, Technology and Research), Salomé LeibundGut‐Landmann(University of Zurich), Michael D. Leipold(Institute of Infection and Immunity), Megan K. Levings(University of British Columbia), Virginia Litwin(Covance (United States)), Yanling Liu(University of Toronto), Michael Lohoff(Philipps University of Marburg), Giovanna Lombardi(Guy's Hospital), Lilly Lopez(Beckman Coulter Foundation), Amy E. Lovett‐Racke(The Ohio State University), Erik Lubberts(Erasmus MC), Burkhard Ludewig(Kantonsspital St. Gallen), Enrico Lugli(Humanitas University), Holden T. Maecker(Institute of Infection and Immunity), Glòria Martrus(Leibniz Institute of Virology (LIV)), Giuseppe Matarese(Institute for Experimental Endocrinology and Oncology), Christian Maueröder(Friedrich-Alexander-Universität Erlangen-Nürnberg), Mairi McGrath(Leibniz Association), Iain B. McInnes(University of Glasgow), Henrik E. Mei(Leibniz Association), Fritz Melchers(Max Planck Institute for Infection Biology), Susanne Melzer(Leipzig University), Dirk Mielenz(Friedrich-Alexander-Universität Erlangen-Nürnberg), Kingston H. G. Mills(Trinity College Dublin), David Mirrer, Jenny Mjösberg(Linköping University), Jonni S. Moore(University of Pennsylvania), Barry Moran(Trinity College Dublin), Alessandro Moretta(University of Genoa), Lorenzo Moretta(Bambino Gesù Children's Hospital), Tim R. Mosmann(University of Rochester Medical Center), Susann Müller(Helmholtz Centre for Environmental Research), Werner Müller(University of Manchester), Christian Münz(University of Zurich), Gabriele Multhoff(TUM Klinikum), Luis E. Muñoz(Friedrich-Alexander-Universität Erlangen-Nürnberg), Kenneth M. Murphy(Howard Hughes Medical Institute), Toshinori Nakayama(Chiba University), Milena Nasi(University of Modena and Reggio Emilia), Christine Neudörfl(Medizinische Hochschule Hannover), John P. Nolan(Scintillon Institute), Sussan Nourshargh(Queen Mary University of London), José‐Enrique O’Connor(Universitat de València), Wenjun Ouyang(Amgen (United States)), Annette Oxenius(ETH Zurich), Raghavendra Palankar(Universitätsmedizin Greifswald), Isabel Panse(University of Oxford), Pärt Peterson(University of Tartu), Christian Peth(Miltenyi Biotec (Germany)), Jordi Pétriz(Josep Carreras Leukaemia Research Institute), Daisy Philips(The Netherlands Cancer Institute), Winfried F. Pickl(Medical University of Vienna), Silvia Piconese(Istituto Pasteur), Marcello Pinti(University of Modena and Reggio Emilia), A. Graham Pockley(Nottingham Trent University), Malgorzata J. Podolska(Friedrich-Alexander-Universität Erlangen-Nürnberg), Carlo Pucillo(University of Udine), Sally A. Quataert(University of Rochester Medical Center), Timothy R. D. J. Radstake(University Medical Center Utrecht), Bartek Rajwa(Purdue University West Lafayette), Jonathan A. Rebhahn(University of Rochester Medical Center), Diether Recktenwald, Ester B. M. Remmerswaal(Amsterdam UMC Location University of Amsterdam), Katy Rezvani(The University of Texas MD Anderson Cancer Center), Laura G. Rico(Josep Carreras Leukaemia Research Institute), J. Paul Robinson(Purdue University West Lafayette), Chiara Romagnani(Leibniz Association), Anna Rubartelli(Ospedale Policlinico San Martino), Beate Rückert, Jürgen Ruland(German Cancer Research Center), Shimon Sakaguchi(Kyoto University), Francisco Sala‐de‐Oyanguren(Universitat de València), Yvonne Samstag(Heidelberg University), Sharon Sanderson(University of Oxford), Birgit Sawitzki(Humboldt-Universität zu Berlin), Alexander Scheffold(Leibniz Association), Matthias Schiemann(Technical University of Munich), Frank A. Schildberg(Harvard University), Esther Schimisky(Miltenyi Biotec (Germany)), Stephan Schmid(University Hospital Regensburg), Steffen Schmitt(German Cancer Research Center), Kilian Schober(Technical University of Munich), Thomas Schüler(Otto-von-Guericke-Universität Magdeburg), Axel Schulz(Leibniz Association), Ton N. Schumacher(The Netherlands Cancer Institute), Cristiano Scottà(Guy's Hospital), T. Vincent Shankey, Anat Shemer(Weizmann Institute of Science), Anna Katharina Simon(University of Oxford), Josef Špidlen(BC Cancer Agency), Alan M. Stall(BD Biosciences (United States)), Regina Stark(Sanquin), Christina Stehle(Leibniz Association), Merle Stein(Friedrich-Alexander-Universität Erlangen-Nürnberg), Tobit Steinmetz(Friedrich-Alexander-Universität Erlangen-Nürnberg), Hannes Stockinger(Medical University of Vienna), Yousuke Takahama(Tokushima University), Attila Tárnok(Fraunhofer Institute for Cell Therapy and Immunology), Zhi‐Gang Tian(University of Science and Technology of China), Gergely Toldi(University of Birmingham), Julia Tornack(Max Planck Institute for Infection Biology), Elisabetta Traggiai(Novartis (Switzerland)), Joe Trotter(BD Biosciences (United States)), Henning Ulrich(Universidade de São Paulo), Marlous van der Braber(The Netherlands Cancer Institute), René A. W. van Lier(Sanquin), Marc Veldhoen(National Legal Medicine Institute), Salvador Vento‐Asturias, Paulo Vieira(Institut Pasteur), David Voehringer(Universitätsklinikum Erlangen), Hans‐Dieter Volk, Konrad von Volkmann, Ari Waisman(Johannes Gutenberg University Mainz), Rachael Walker(Babraham Institute), Michael D. Ward(Thermo Fisher Scientific (United States)), Klaus Warnatz(University of Freiburg), Sarah Warth(Berlin-Brandenburger Centrum für Regenerative Therapien), James Watson, Carsten Watzl(TU Dortmund University), Leonie Wegener(Miltenyi Biotec (Germany)), Annika Wiedemann(Charité - Universitätsmedizin Berlin), Jürgen Wienands(Universitätsmedizin Göttingen), Gerald Willimsky(German Cancer Research Center), James B. Wing(Kyoto University), Peter Wurst(University of Bonn), Liping Yu(BD Biosciences (United States)), Alice Yue(Simon Fraser University), Qianjun Zhang, Yi Zhao(Sichuan University), Susanne Ziegler(Leibniz Institute of Virology (LIV)), Jakob Zimmermann(University of Bern)
Cited by 547Open Access
Abstract
Funding Information: Mairi Mc Grath and Regina Stark thank Francesco Siracusa and Patrick Maschmeyer for providing data and Klaas van Gisbergen for helpful discussions. Philip E. Boulais and Paul S. Frenette are grateful to Dr. Sandra Pinho for helpful comments and suggestions. They thank the National Institutes of Health for their support (R01 grants DK056638, HL116340, HL097819 to P.S.F). They also thank the New York State Department of Health (NYSTEM Program) for shared facility (C029154) and research support (N13G-262) and the Leukemia and Lymphoma Society’s Translational Research Program. Funding Information: Acknowledgements: Enrico Lugli and Pratip K. Chattopadhyay were supported by grants from the Fondazione Cariplo (Grant Ricerca Biomedica 2012/0683), the Italian Ministry of Health (Bando Giovani Ricercatori GR-2011-02347324) and the European Union Marie Curie Career Integration Grant 322093 (all to E.L.). E.L. and P.K.C. are International Society for the Advancement of Cytometry (ISAC) Marylou Ingram scholars. Alice Yue and Ryan R. Brinkman were funded by Genome BC and NSERC. Klaus Warnatz received funding from the German Federal Ministry of Education and Research (BMBF 01EO1303) and the Deutsche Forschungsgemeinschaft (DECIDE, DFG WA 1597/4-1 and the TRR130). The Jung laboratory is supported by funds of the ERC and ISF. Henrik Mei is a 2017-2021 ISAC scholar. Antonio Cosma is supported by the French government program: “Investissement d’avenir: Equipements d’Excellence” (EQUIPEX)-2010 FlowCyTech, Grant number: ANR-10-EQPX-02-01. Henrik Mei is supported by the Deutsche Forschungsgemeinschaft (DFG; grants Me3644/5-1 and TRR130/TP24). Funding Information: The Immunology Database and Analysis Portal (ImmPort) system provides an archive of immunology research data generated by investigators mainly funded through the National Institutes of Health (NIH), National Institute of Allergy and Infectious Diseases (NIAID), Division of Allergy, Immunology, and Transplantation (DAIT). It is an extensive data warehouse containing an integration of experimental and clinical trial data generated by dozens of assay types, including 63 flow cytometry and 5 CyTOF data sets. In addition, the ImmPort system also provides data analysis tools and it contains implicit knowledge and ‘‘best practices’’ for clinical and genomic studies in the form of nearly 50 templates for data deposition, management, and dissemination. ImmPort has been developed under the Bioinformatics Integration Support Contract (BISC) by the Northrop Grumman Information Technology Health
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