Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity
Stanislav S. Terekhov(Lomonosov Moscow State University), I. V. Smirnov(Kazan Federal University), Anastasiya Stepanova(Institute of Bioorganic Chemistry), Tatyana V. Bobik(Kazan Federal University), Yuliana A. Mokrushina(Institute of Bioorganic Chemistry), Н. А. Пономаренко(Institute of Bioorganic Chemistry), Alexey A. Belogurov(Kazan Federal University), Maria P. Rubtsova(Skolkovo Institute of Science and Technology), Olga V. Kartseva(Kazan Federal University), Marina Gomzikova(Kazan Federal University), Alexey A. Moskovtsev(Research Institute of General Pathology and Pathophysiology, the Russian Academy of Medical Sciences), Anton Bukatin(Saint Petersburg Academic University), Michael Dubina(Saint Petersburg Academic University), Elena Kostryukova(Moscow Institute of Physics and Technology), Vladislav V. Babenko(Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin), Maria T Vakhitova(Moscow Institute of Physics and Technology), Alexander I. Manolov(Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin), Maja V. Malakhova(Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin), Maria Kornienko(Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin), Alexander Tyakht(Moscow Institute of Physics and Technology), Anna Vanyushkina(Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin), Elena N. Ilina(Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin), Patrick Masson(Kazan Federal University), Alexander G. Gabibov(Lomonosov Moscow State University), Sidney Altman(Yale University)
Cited by 230Open Access
Abstract
Significance Biocompatible microfluidic double water-in-oil-in-water emulsion (MDE) enables in-droplet cultivation of different living species. The combination of droplet-generating machinery with FACS followed by next-generation sequencing and liquid chromatography-mass spectrometry analysis of the secretomes of encapsulated organisms yielded detailed genotype/phenotype descriptions. The MDE–FACS platform we developed enabled highly sensitive single-cell selection of predesigned activity and exploration of pairwise interactions between target and effector cells without interference from other microbiota species.
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