Derivation and characterization of the human embryonic stem cell line CR-4: Differentiation to human retinal pigment epithelial cells
John L. Mazzilli(Brown Foundation), Aleksey Y. Domozhirov(The University of Texas Health Science Center at Houston), Stacey L. Mueller‐Ortiz(The University of Texas Health Science Center at Houston), Charles García(The University of Texas Health Science Center at Houston), Rick A. Wetsel(The University of Texas Health Science Center at Houston), Eva Zsigmond(The University of Texas Health Science Center at Houston)
Cited by 6Open Access
Abstract
The CR-4 human embryonic stem cell line was derived from the inner cell mass of a developing blastocyst. This cell line has been adapted to grow in feeder-free conditions and is especially well-suited for differentiation to retinal pigment epithelium. The line demonstrates a normal human 46,XX female karyotype. Pluripotency was assessed through qRT-PCR for expression of NANOG, OCT-4, and SOX-2. A teratoma assay was performed and results were positive for all three germ layers. Testing for Mycoplasma was negative.
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