Consequences of Sox2 over-expression in the wound healing <i>in vitro</i> assay.

Abstract

<p><b>A.</b> Pictures were acquired at the beginning of the experiment (t = 0, immediately after wounding) and from the same field at the end of the experiment (t = 24 h for this example from the Calu-1 cell line). <b>B.</b> Quantification of wound closure for the three cell lines (BEAS-2B, NCI-H226 and Calu-1). Wound sizes were measured at the beginning and end of the experiment to calculate the percentage of wound closure for control and SOX2 over-expressing cells for each of the three cell lines. SOX2 over-expression significantly stimulates cell migration compared to control cells (student's t-test).</p>