Direct Conversion of Fibroblasts to Megakaryocyte Progenitors
Julián Pulecio(Barcelona Biomedical Research Park), Oriol Alejo-Valle(Barcelona Biomedical Research Park), Sandra Capellera-Garcia(Lund University), Marianna Vitaloni(Barcelona Biomedical Research Park), Paula Rı́o(Instituto de Salud Carlos III), Eva Mejía-Ramírez(Barcelona Biomedical Research Park), Ilaria Caserta(Barcelona Biomedical Research Park), Juan A. Bueren(Instituto de Salud Carlos III), Johan Flygare(Lund University), Ángel Raya(Institució Catalana de Recerca i Estudis Avançats)
Cited by 32Open Access
Abstract
, display polylobulated nuclei, have ploidies higher than 4N, form MK colonies, and give rise to platelets in vitro. Moreover, transplantation of MK-like murine progenitor cells into NSG mice results in successful engraftment and further maturation in vivo. Similar results are obtained using disease-corrected fibroblasts from Fanconi anemia patients. Our results combined demonstrate that functional MK progenitors with clinical potential can be obtained in vitro, circumventing the use of hematopoietic progenitors or pluripotent stem cells.
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