Alpha‐synuclein <scp>RT</scp>‐Qu<scp>IC</scp> in the <scp>CSF</scp> of patients with alpha‐synucleinopathies

Graham Fairfoul(Western General Hospital), Lynne I. McGuire(Western General Hospital), Suvankar Pal(Western General Hospital), James W. Ironside(Western General Hospital), Juliane Neumann(John Radcliffe Hospital), Sharon Christie(John Radcliffe Hospital), Catherine Joachim(John Radcliffe Hospital), Margaret M. Esiri(John Radcliffe Hospital), Samuel Evetts(John Radcliffe Hospital), Michal Rolinski(John Radcliffe Hospital), Fahd Baig(John Radcliffe Hospital), Claudio Ruffmann(John Radcliffe Hospital), Richard Wade‐Martins(University of Oxford), Michele T. M. Hu(John Radcliffe Hospital), Laura Parkkinen(John Radcliffe Hospital), Alison J. E. Green(Western General Hospital)
Annals of Clinical and Translational Neurology
August 28, 2016
10.1002/acn3.338
Cited by 611Open Access
Full Text

Abstract

We have developed a novel real-time quaking-induced conversion RT-QuIC-based assay to detect alpha-synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha-synuclein aggregation in Dementia with Lewy bodies and Parkinson's disease cerebrospinal fluid with sensitivities of 92% and 95%, respectively, and with an overall specificity of 100% when compared to Alzheimer and control cerebrospinal fluid. Patients with neuropathologically confirmed tauopathies (progressive supranuclear palsy; corticobasal degeneration) gave negative results. These results suggest that RT-QuiC analysis of cerebrospinal fluid is potentially useful for the early clinical assessment of patients with alpha-synucleinopathies.

Related Papers

No related papers found

Powered by citation graph analysis