andHerpes-Type Virus Particles intheP3HR-1Burkitt LymphomaCellLine

Abstract

was similar or identical tothatfoundinseveral other Burkitt tumorcell lines inprevious studies. Whenthecells wereincubated at 35or 32C for9to15dayswithout refeeding, more than50%ofthecells became immunofluorescence-positive. Thirteen different cultures ofP3HR-1cells, which contained up to75%immunofluorescence-positive cells, were thin-sectioned and examined byelectron microscopy. Thepercentage ofcells containing herpes-type virus particles inthecultures varied from<3to78%.There was generally a good correlation between thenumberofimmunofluorescent cells andthenumberofcells containing virus particles. Thenumberofvirus particles percell section ranged from 1 tomore than100. Theseresults strongly support thehypothesis thattheimmunofluorescent antigen isrelated tothepresenceoftheherpes-type virus particle inthe cells.