Molecular Beacons: A Novel Approach to Detect Protein - DNA Interactions

Abstract

Through clever design of an oligonucleotide probe, the molecular beacon (MB), protein – DNA interactions can be studied and proteins quantified. The principle involves a design in which the fluorophore (F) of the MB is quenched by intramolecular interaction with a quenching group (Q) in close proximity. This quenching is relieved by the interaction of the molecular beacon with a single-stranded DNA binding protein (SSB). As a result, fluorescence is restored and can be monitored. This approach could be developed into a powerful method for directly monitoring protein production in living cells and organs.