Visualization of RNA-Quadruplexes in Live Cells
Aurélien Laguerre(Université de Bourgogne), Kyle R. Hukezalie(University of British Columbia), Pascale Winckler(Université de Bourgogne), Fares Katranji(Université de Bourgogne), Gaëtan Chanteloup(Centre National de la Recherche Scientifique), Marc Pirrotta(Centre National de la Recherche Scientifique), Jean‐Marie Perrier‐Cornet(Université de Bourgogne), Judy M.Y. Wong(University of British Columbia), David Monchaud(Centre National de la Recherche Scientifique)
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Abstract
Visualization of DNA and RNA quadruplex formation in human cells was demonstrated recently with different quadruplex-specific antibodies. Despite the significant interest in these immunodetection approaches, dynamic detection of quadruplex in live cells remains elusive. Here, we report on NaphthoTASQ (N-TASQ), a next-generation quadruplex ligand that acts as a multiphoton turn-on fluorescent probe. Single-step incubation of human and mouse cells with N-TASQ enables the direct detection of RNA-quadruplexes in untreated cells (no fixation, permeabilization or mounting steps), thus offering a unique, unbiased visualization of quadruplexes in live cells.
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