[Antigen mimicry of anti-idiotypic antibodies for human ovarian carcinoma antigen: generation and characterization of anti anti-idiotype antibodies].

Abstract

The antigen mimicry of monoclonal anti-idiotypic antibodies 6B11 and 1H12 was investigated, which carrying the internal image of human ovarian carcinoma antigen. Anti-anti-idiotypic antibodies, obtained from the mice immunized with 6B11 or 1H12, binded to ovarian tumor antigen OC 166-9 specifically by enzyme linked immunosorbent assay (ELISA). Western blot analysis showed that Ab3 and Ab1 recognized the same antigenic protein. Through antibody dependent cell-mediated cytotoxicity (ADCC), Ab3 sera killed the ovarian carcinoma cell line SKOV3 specifically in vitro. These results suggest that 6B11 and 1H12 may substitute for the nominal antigen to stimulate a specific immune response and that they are potential candidates as idiotype vaccines against ovarian carcinomas.