Cerebrospinal fluid real‐time quaking‐induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: An international study
Lynne I. McGuire(Western General Hospital), Anna Poleggi(Institute of Neurological Sciences), Ilaria Poggiolini(Institute of Neurological Sciences), Silvia Suardi(Fondazione IRCCS Istituto Neurologico Carlo Besta), Katarína Grznárová(Sorbonne Université), Song Shi(Ludwig-Maximilians-Universität München), Bart De Vil(University of Antwerp), Shannon Sarros(The University of Melbourne), Katsuya Satoh(Nagasaki University), Keding Cheng(Public Health Agency of Canada), Maria Cramm(German Center for Neurodegenerative Diseases), Graham Fairfoul(Western General Hospital), Matthias Schmitz(German Center for Neurodegenerative Diseases), Inga Zerr(German Center for Neurodegenerative Diseases), Patrick Cras(University of Antwerp), Michele Equestre(Institute of Neurological Sciences), Fabrizio Tagliavini(Fondazione IRCCS Istituto Neurologico Carlo Besta), Ryuichiro Atarashi(Nagasaki University), David Knox(Public Health Agency of Canada), Steven Collins(The University of Melbourne), Stéphane Haı̈k(Sorbonne Université), Piero Parchi(Institute of Neurological Sciences), Maurizio Pocchiari(Institute of Neurological Sciences), Alison Green(Western General Hospital)
Cited by 125Open Access
Abstract
Real-time quaking-induced conversion (RT-QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt-Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be demonstrated. Two international ring trials were undertaken in which a set of 25 cerebrospinal fluid samples were analyzed by a total of 11 different centers using a range of recombinant prion protein substrates and instrumentation. The results show almost complete concordance between the centers and demonstrate that RT-QuIC is a suitably reliable and robust technique for clinical practice. Ann Neurol 2016;80:160-165.
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