High-yield isolation of extracellular vesicles using aqueous two-phase system
Hyun‐Woo Shin(Pohang University of Science and Technology), Chungmin Han(Pohang University of Science and Technology), Joseph M. Labuz(University of Michigan), Jiyoon Kim(Pohang University of Science and Technology), Jongmin Kim(Pohang University of Science and Technology), Siwoo Cho(Pohang University of Science and Technology), Yong Song Gho(Pohang University of Science and Technology), Shuichi Takayama(University of Michigan), Jaesung Park(Pohang University of Science and Technology)
Cited by 158Open Access
Abstract
Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs as diagnostic biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne EVs have been hindered by the lack of effective purification strategies. In this study, an efficient EV isolation method was developed by using polyethylene glycol/dextran aqueous two phase system (ATPS). This method provides high EV recovery efficiency (~70%) in a short time (~15 min). Consequently, it can significantly increase the diagnostic applicability of EVs.
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