PHYLOGENETIC RELATIONSHIPS BETWEEN MONACROSPOIUM EUDERMATUM AND ARTHROBOTRYS MUSIFORMIS REVEALED BY RAPD

Abstract

Seven isolates of Arthrobotrys musiformis and nine isolates of Monacrosporium eudermatum from Guizhou, Guangxi, Hunan, Beijing, Yunnan and Anhui were characterized by RAPD (Randomly Amplified Polymorphic DNA) analysis with four 10-mer arbitrary primers (OPW-18, OPW-16, OPM-1, OPK-19). Computer analysis of the data showed that RAPD analysis did not distinguish between Arthrobotrys and Monacrosporium. The isolates with appendage did not get together,and the phylogeny among isolates did not exhibit their geographical origin and habitat.The result suggested that this method is not a useful tool for identification at the generic level of nematophagous fungi, and appendage is unimportant character on taxonomy. The establishment of Drechslerella is unreasonable, because the isolates with or without appendage obtrined from different regions and habtats show very similar RAPD profiles.