Genetic Diversity Analysis of Medicago ruthenica by ISSRs in China

Abstract

The genetic relationship of M.ruthenica populations from seven provinces of China were analyzed by ISSR marker.16 primers with strong polymorphism and good repeatability were screened from 80 ISSR primers.133 bands were amplified from 44 populations M.ruthenica genomic DNA,with an average of 8.31 bands in each primer,of which(115 bands)87.08% were polymorphic.The genetic dissimilarity coefficients of M.ruthenica germplasm ranged from 0.436 to 0.908.44 populations M.ruthenica germplasm were divided into eight groups with the higher correlation of geography resource by UPGMA cluster analysis.