Isolation of circulating tumor cells using CellCelector post CellSearch enables characterization of single cells

Martín Neumann(Universitäts Frauenklinik), S Schömer(Heinrich Heine University Düsseldorf), Y Decker(Düsseldorf University Hospital), H Schneck(Düsseldorf University Hospital), M Fleisch(Heinrich Heine University Düsseldorf), Rui P. Neves(Heinrich Heine University Düsseldorf), N Stoecklein(Heinrich Heine University Düsseldorf), Tanja Fehm(Heinrich Heine University Düsseldorf), F Meier-Stiegen(Düsseldorf University Hospital), Hans Neubauer(Heinrich Heine University Düsseldorf), Dieter Niederacher(Düsseldorf University Hospital)
Senologie - Zeitschrift für Mammadiagnostik und -therapie
May 22, 2015
Cited by 1

Abstract

Circulating tumor cells (CTC) are rare cells and dissociated successfully from the primary tumor into the blood stream. Only a small proportion is capable to extravasate and form metastases. The presence of CTCs is associated with increased risk to develop metastases. Understanding the heterologous CTC biology enables the choice of the optimal therapy and improves the prognosis. Therefore, detection and isolation of CTCs is necessary. Here we show a workflow to characterize CTCs by combining the advantages of both the CellSearch®- and CellCelector™-System.


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