One-Step Cloning and Chromosomal Integration of DNA
François St-Pierre, Lun Cui(University of Adelaide), David G. Priest(University of Adelaide), Drew Endy, Ian B. Dodd(University of Adelaide), Keith E. Shearwin(University of Adelaide)
Cited by 239
Abstract
We describe "clonetegration", a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.
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