Expression analysis of the macrophage-capping protein (CapG) in single circulating tumor cells (CTCs) isolated from blood samples of breast cancer patients
Y Decker(Heinrich Heine University Düsseldorf), M Neumann(Heinrich Heine University Düsseldorf), Markus C. Fleisch(Heinrich Heine University Düsseldorf), H Neubauer(Heinrich Heine University Düsseldorf), Dieter Niederacher(Heinrich Heine University Düsseldorf), Tanja Fehm(Heinrich Heine University Düsseldorf)
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Abstract
Results: Detection and isolation of single EPCAM+ as well as EPCAM- cells was successfully established using blood samples spiked with low cell numbers of known BC cell lines displaying different EPCAM expression. CellSelector re-analysis of CellSearch cartridges revealed comparable CTC detection rates. EPCAM-/CK+ cells were successfully recovered from CellSearch "waste" samples by size selection and isolated from filter membranes by micromanipulation using CellSelector. CapG
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