Allozyme polymorphisms detected in mature needle tissue of ponderosa pine

Abstract

A method for extraction of enzymes from mature leaves of a diversity of trees is described. The methods of tissue preparation allow samples to be collected at any time of year regardless of the reproductive state of the plant. We have used this method to detect six enzyme polymorphisms in ponderosa pine and we present evidence to show that the genes coding for these proteins are simple Mendelian loci.