HIV-1 infection and virus production in follicular dendritic cells in lymph nodes. A case report, with analysis of isolated follicular dendritic cells.

Abstract

Follicular dendritic cells (FDC) from axillary lymphoid tissue of a patient with acquired immune deficiency syndrome (AIDS) were analyzed for the presence of gag and env proteins and env mRNA of human immunodeficiency virus type-1 (HIV-1), both in a purified FDC suspension and on frozen sections. Isolated cells with morphologic and immunocytochemical features of FDC expressed HIV-1 core (gag) proteins p15, p17, p24, and envelope (env) protein gp41; in addition HIV-1 env mRNA was detected in some of these cells. This corresponded with intense expression of HIV-1 proteins by FDC in germinal centers in situ, and the presence of HIV-1 mRNA-positive cells in germinal follicles. These findings led us to conclude that FDC are infected and able to produce HIV-1. Such infection may contribute significantly to the destruction of the FDC network during the lymphadenopathy phase after HIV-1 infection.