MALAT1 long ncRNA promotes gastric cancer metastasis by suppressing <i>PCDH10</i>

Abstract

// Ying Qi 1 , Hong Sain Ooi 2 , Jun Wu 3 , Jian Chen 1 , Xiaoli Zhang 1 , Sheng Tan 4 , Qing Yu 4 , Yuan-Yuan Li 5, 6 , Yani Kang 1 , Hua Li 1 , Zirui Xiong 4 , Tao Zhu 4 , Bingya Liu 1, 7 , Zhifeng Shao 1 , Xiaodong Zhao 1 1 School of Biomedical Engineering, Bio-ID Research Center, State Key Laboratory for Oncogenes and Related Genes, Shanghai Jiao Tong University, Shanghai, China 2 Department of Biomedicine, Aarhus University, Aarhus, Denmark 3 Department of Automation, Shanghai Jiao Tong University, Shanghai, China 4 Laboratory of Molecular Tumor Pathology, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China 5 Shanghai Center for Bioinformatics Technology, Shanghai, China 6 Shanghai Engineering Research Center of Pharmaceutical Translation, Shanghai, China 7 Department of Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China Correspondence to: Bingya Liu, e-mail: bingyaliu@sjtu.edu.cn Xiaodong Zhao, e-mail: xiaodongzhao@sjtu.edu.cn Keywords: EZH2, RIP-seq, MALAT1, gastric cancer, transcriptional silencing Received: September 29, 2015&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Accepted: January 23, 2016&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Published: February 09, 2016 ABSTRACT EZH2, the catalytic component of polycomb repressive complex 2 (PRC2), is frequently overexpressed in human cancers and contributes to tumor initiation and progression, in part through transcriptional silencing of tumor suppressor genes. A number of noncoding RNAs (ncRNAs) recruit EZH2 to specific chromatin loci, where they modulate gene expression. Here, we used RNA immunoprecipitation sequencing (RIP-seq) to profile EZH2-associated transcripts in human gastric cancer cell lines. We identified 8,256 transcripts, including both noncoding and coding transcripts, some of which were derived from cancer-related loci. In particular, we found that long noncoding RNA (lncRNA) MALAT1 binds EZH2, suppresses the tumor suppressor PCDH10 , and promotes gastric cellular migration and invasion. Our work thus provides a global view of the EZH2-associated transcriptome and offers new insight into the function of EZH2 in gastric tumorigenesis.