TLR3 stimulation activates a JAK2/STAT1 pathway in hMSC.

Abstract

<p>(A) Human MSC gene induction, following 6 hours of poly(I:C) treatment, was analyzed by qPCR using 5 separate donors. Data are presented as mean ± SEM. Significance was determined by comparing the poly(I:C) treated samples to the untreated control. *p<0.05. (B-C) JAK2/STAT1 phosphorylation was analyzed by Western blot following TLR3 stimulation for 0–6 hours. Of note, the monoclonal STAT1 antibody preferentially binds total STAT1, which is why total STAT1 expression appears inversely proportional to phosphorylated STAT1. Densitometry was determined by subtracting overall background, then each experimental band was normalized to the actin loading control band within its lane, and fold change was calculated based upon the untreated control band. Density values below each band are representative of results from 3 separate donors.</p>