PICH promotes sister chromatid disjunction and co-operates with topoisomerase II in mitosis

Christian Friberg Nielsen(University of Copenhagen), Diana Huttner(University of Copenhagen), Anna H. Bizard(University of Copenhagen), Seiki Hirano(John Radcliffe Hospital), Tian‐Neng Li(National Tsing Hua University), Timea Palmai‐Pallag(John Radcliffe Hospital), Victoria A. Bjerregaard(University of Copenhagen), Ying Liu(University of Copenhagen), Erich A. Nigg(University of Basel), Lily Wang(University of Basel), Ian D. Hickson(University of Copenhagen)
Nature Communications
December 8, 2015
10.1038/ncomms9962
Cited by 131Open Access
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Abstract

PICH is a SNF2 family DNA translocase that binds to ultra-fine DNA bridges (UFBs) in mitosis. Numerous roles for PICH have been proposed from protein depletion experiments, but a consensus has failed to emerge. Here, we report that deletion of PICH in avian cells causes chromosome structural abnormalities, and hypersensitivity to an inhibitor of Topoisomerase II (Topo II), ICRF-193. ICRF-193-treated PICH(-/-) cells undergo sister chromatid non-disjunction in anaphase, and frequently abort cytokinesis. PICH co-localizes with Topo IIα on UFBs and at the ribosomal DNA locus, and the timely resolution of both structures depends on the ATPase activity of PICH. Purified PICH protein strongly stimulates the catalytic activity of Topo II in vitro. Consistent with this, a human PICH(-/-) cell line exhibits chromosome instability and chromosome condensation and decatenation defects similar to those of ICRF-193-treated cells. We propose that PICH and Topo II cooperate to prevent chromosome missegregation events in mitosis.

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