Genetically Programmed In Vivo Packaging of Protein Cargo and Its Controlled Release from Bacteriophage P22
Alison O’Neil(Montana State University), Courtney Reichhardt(Montana State University), Benjamin C. Johnson(Montana State University), Peter E. Prevelige(University of Alabama at Birmingham), Trevor Douglas(Montana State University)
Cited by 172
Abstract
Packed and ready to go: A scaffold protein (SP) aids the assembly of Salmonella typhimuriam bacteriophage P22 into a capsid, with encapsulation of the SP. This natural process was exploited by using an engineered molecular system to fuse a fluorescent protein cargo (green in the picture) to a portion of the SP (yellow), which templated accurate spontaneous assembly. Heating of the capsids and treatment with thrombin released the SP but not the cargo.
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