CD1d-Restricted and TCR-Mediated Activation of V <sub>α</sub> 14 NKT Cells by Glycosylceramides
Tetsu Kawano(Kirin (Japan)), Junqing Cui(Kirin (Japan)), Yasuhiko Koezuka(Kirin (Japan)), Isao Toura(Kirin (Japan)), Yoshikatsu Kaneko(Kirin (Japan)), Kazuhiro Motoki(Kirin (Japan)), Hitomi Ueno(Kirin (Japan)), Ryusuke Nakagawa(Kirin (Japan)), Hiroshi Sato(Kirin (Japan)), Eisuke Kondo(Kirin (Japan)), Haruhiko Koseki(Kirin (Japan)), Masaru Taniguchi(Kirin (Japan))
Cited by 2,389
Abstract
Natural killer T (NKT) lymphocytes express an invariant T cell antigen receptor (TCR) encoded by the Valpha14 and Jalpha281 gene segments. A glycosylceramide-containing alpha-anomeric sugar with a longer fatty acyl chain (C26) and sphingosine base (C18) was identified as a ligand for this TCR. Glycosylceramide-mediated proliferative responses of Valpha14 NKT cells were abrogated by treatment with chloroquine-concanamycin A or by monoclonal antibodies against CD1d/Vbeta8, CD40/CD40L, or B7/CTLA-4/CD28, but not by interference with the function of a transporter-associated protein. Thus, this lymphocyte shares distinct recognition systems with either T or NK cells.