The genome sequence of the facultative intracellular pathogen <i>Brucella melitensis</i>

Vito G. DelVecchio(Institut de Neurobiologie de la Méditerranée), Vinayak Kapatral(Institut de Neurobiologie de la Méditerranée), Rajendra J. Redkar(Institut de Neurobiologie de la Méditerranée), Guy Patra(Institut de Neurobiologie de la Méditerranée), Cesar V. Mujer(Institut de Neurobiologie de la Méditerranée), Tamara Los(Institut de Neurobiologie de la Méditerranée), Natalia Ivanova(Institut de Neurobiologie de la Méditerranée), Iain Anderson(Institut de Neurobiologie de la Méditerranée), Anamitra Bhattacharyya(Institut de Neurobiologie de la Méditerranée), Athanasios Lykidis(Institut de Neurobiologie de la Méditerranée), Gary Reznik(Institut de Neurobiologie de la Méditerranée), L Jabłoński(Institut de Neurobiologie de la Méditerranée), Niels Larsen(Institut de Neurobiologie de la Méditerranée), Mark D’Souza(Institut de Neurobiologie de la Méditerranée), Axel Bernal(Institut de Neurobiologie de la Méditerranée), Mikhail Mazur(Institut de Neurobiologie de la Méditerranée), Eugene Goltsman(Institut de Neurobiologie de la Méditerranée), Eugene Selkov(Institut de Neurobiologie de la Méditerranée), Philip H. Elzer(Institut de Neurobiologie de la Méditerranée), Sue D. Hagius(Institut de Neurobiologie de la Méditerranée), David O’Callaghan(Institut de Neurobiologie de la Méditerranée), Jean‐Jacques Letesson(Institut de Neurobiologie de la Méditerranée), Robert Haselkorn(Institut de Neurobiologie de la Méditerranée), Nikos C. Kyrpides(Institut de Neurobiologie de la Méditerranée), Ross Overbeek(Institut de Neurobiologie de la Méditerranée)
Proceedings of the National Academy of Sciences
December 26, 2001
10.1073/pnas.221575398
Cited by 548Open Access
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Abstract

Brucella melitensis is a facultative intracellular bacterial pathogen that causes abortion in goats and sheep and Malta fever in humans. The genome of B. melitensis strain 16M was sequenced and found to contain 3,294,935 bp distributed over two circular chromosomes of 2,117,144 bp and 1,177,787 bp encoding 3,197 ORFs. By using the bioinformatics suite ERGO, 2,487 (78%) ORFs were assigned functions. The origins of replication of the two chromosomes are similar to those of other alpha-proteobacteria. Housekeeping genes, including those involved in DNA replication, transcription, translation, core metabolism, and cell wall biosynthesis, are distributed on both chromosomes. Type I, II, and III secretion systems are absent, but genes encoding sec-dependent, sec-independent, and flagella-specific type III, type IV, and type V secretion systems as well as adhesins, invasins, and hemolysins were identified. Several features of the B. melitensis genome are similar to those of the symbiotic Sinorhizobium meliloti.

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