Mesenchymal Stem Cell-Mediated Functional Tooth Regeneration in Swine

Wataru Sonoyama(Okayama University), Yi Liu(Capital Medical University), Dianji Fang(Capital Medical University), Takayoshi Yamaza(University of Southern California), Byoung‐Moo Seo(Seoul National University Dental Hospital), Chunmei Zhang(Capital Medical University), He Liu(Peking University), Stan Gronthos(South Australia Pathology), Cun-Yu Wang(Michigan United), Songtao Shi(Capital Medical University), Songlin Wang(University of Southern California)
PLoS ONE
December 20, 2006
Cited by 1,350Open Access
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Abstract

Mesenchymal stem cell-mediated tissue regeneration is a promising approach for regenerative medicine for a wide range of applications. Here we report a new population of stem cells isolated from the root apical papilla of human teeth (SCAP, stem cells from apical papilla). Using a minipig model, we transplanted both human SCAP and periodontal ligament stem cells (PDLSCs) to generate a root/periodontal complex capable of supporting a porcelain crown, resulting in normal tooth function. This work integrates a stem cell-mediated tissue regeneration strategy, engineered materials for structure, and current dental crown technologies. This hybridized tissue engineering approach led to recovery of tooth strength and appearance.


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