A J-protein is an essential subunit of the presequence translocase–associated protein import motor of mitochondria

Kaye N. Truscott(University of Freiburg), Wolfgang Voos(University of Freiburg), Ann E. Frazier(University of Freiburg), Maria Lind(University of Freiburg), Yanfeng Li(University of Freiburg), Andreas Geissler(University of Freiburg), Jan Dudek(University of Freiburg), Hanne Müller(University of Freiburg), Albert Sickmann(University of Würzburg), Helmut E. Meyer(Ruhr University Bochum), Chris Meisinger(University of Freiburg), Bernard Guiard(Centre National de la Recherche Scientifique), Peter Rehling(University of Freiburg), Nikolaus Pfanner(University of Freiburg)
The Journal of Cell Biology
November 24, 2003
10.1083/jcb.200308004
Cited by 225Open Access
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Abstract

Transport of preproteins into the mitochondrial matrix is mediated by the presequence translocase-associated motor (PAM). Three essential subunits of the motor are known: mitochondrial Hsp70 (mtHsp70); the peripheral membrane protein Tim44; and the nucleotide exchange factor Mge1. We have identified the fourth essential subunit of the PAM, an essential inner membrane protein of 18 kD with a J-domain that stimulates the ATPase activity of mtHsp70. The novel J-protein (encoded by PAM18/YLR008c/TIM14) is required for the interaction of mtHsp70 with Tim44 and protein translocation into the matrix. We conclude that the reaction cycle of the PAM of mitochondria involves an essential J-protein.

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