Reconstitution of the Entire Hepatitis C Virus Life Cycle in Nonhepatic Cells
Daniel Da Costa(Inserm), Marine Turek(Inserm), Daniel J. Felmlee(Inserm), Erika Girardi(Centre National de la Recherche Scientifique), Sébastien Pfeffer(Centre National de la Recherche Scientifique), Gang Long(Heidelberg University), Ralf Bartenschlager(Heidelberg University), Mirjam B. Zeisel(Inserm), Thomas F. Baumert(Inserm)
Cited by 94Open Access
Abstract
Hepatitis C virus (HCV) is a human hepatotropic virus, but the relevant host factors restricting HCV infection to hepatocytes are only partially understood. We demonstrate that exogenous expression of defined host factors reconstituted the entire HCV life cycle in human nonhepatic 293T cells. This study shows robust HCV entry, RNA replication, and production of infectious virus in human nonhepatic cells and highlights key host factors required for liver tropism of HCV.
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