Microbial Transformation and Transfection

Abstract

FORMATION OF COMPETENCE STATE 375 Physiological state of culture.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 375 Evidence for wall changes accompanying competence . . . . . . . . . . . . . . . . . . . .. 377 EVIDENCE FOR A DNA RECEPTOR OR COMPETENCE FACTOR. 378 CHARACTERISTICS OF BIOLOGICALLY ACTIVE DNA. 379 Molecular size . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 379 Strandedness. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 381 DNA INTEGRATION IN TRANSFORMATION: FATE OF TRANSFORMING DNA 382 INTEGRATION OF ONE OR Two STRANDS: THE PROBLEM OF HETEROZYGOTES 385 LINKAGE GROUPS AND MAPPING OF GENOME BY TRANSFORMATION .. . 386 RNA TRANSFORMATION 389 TRANSFECTION. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 390