A Riboswitch Regulates Expression of the Coenzyme B <sub>12</sub> -Independent Methionine Synthase in <i>Mycobacterium tuberculosis</i> : Implications for Differential Methionine Synthase Function in Strains H37Rv and CDC1551
Digby F. Warner(National Health Laboratory Service), Suzana Savvi(National Health Laboratory Service), Valerie Mizrahi(National Health Laboratory Service), Stephanie S. Dawes(National Health Laboratory Service)
Cited by 89
Abstract
We observed vitamin B(12)-mediated growth inhibition of Mycobacterium tuberculosis strain CDC1551. The B(12) sensitivity was mapped to a polymorphism in metH, encoding a coenzyme B(12)-dependent methionine synthase. Vitamin B(12)-resistant suppressor mutants of CDC1551 containing mutations in a B(12) riboswitch upstream of the metE gene, which encodes a B(12)-independent methionine synthase, were isolated. Expression analysis confirmed that the B(12) riboswitch is a transcriptional regulator of metE in M. tuberculosis.
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