Frequent deletions and down-regulation of micro- RNA genes <i>miR15</i> and <i>miR16</i> at 13q14 in chronic lymphocytic leukemia

George A. Calin(Thomas Jefferson University), Calin Dan Dumitru(Thomas Jefferson University), Masayoshi Shimizu(Thomas Jefferson University), Roberta Bichi(Thomas Jefferson University), Simona Zupo(Thomas Jefferson University), Evan Noch(Thomas Jefferson University), Hansjuerg Aldler(Thomas Jefferson University), Sashi Rattan(Thomas Jefferson University), Michael J. Keating(Thomas Jefferson University), Kanti Rai(Thomas Jefferson University), Laura Z. Rassenti(Thomas Jefferson University), Thomas J. Kipps(Thomas Jefferson University), Massimo Negrini(Thomas Jefferson University), Florencia Bullrich(Thomas Jefferson University), Carlo M. Croce(Thomas Jefferson University)
Proceedings of the National Academy of Sciences
November 14, 2002
10.1073/pnas.242606799
Cited by 5,015Open Access
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Abstract

Micro-RNAs (miR genes) are a large family of highly conserved noncoding genes thought to be involved in temporal and tissue-specific gene regulation. MiRs are transcribed as short hairpin precursors ( approximately 70 nt) and are processed into active 21- to 22-nt RNAs by Dicer, a ribonuclease that recognizes target mRNAs via base-pairing interactions. Here we show that miR15 and miR16 are located at chromosome 13q14, a region deleted in more than half of B cell chronic lymphocytic leukemias (B-CLL). Detailed deletion and expression analysis shows that miR15 and miR16 are located within a 30-kb region of loss in CLL, and that both genes are deleted or down-regulated in the majority ( approximately 68%) of CLL cases.

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