Fluorescence Visualization of Newly Synthesized Proteins in Mammalian Cells
Kimberly E. Beatty(California Institute of Technology), Julie C. Liu(California Institute of Technology), Fang Xie(University of South Carolina), Daniela C. Dieterich(California Institute of Technology), Erin M. Schuman(California Institute of Technology), Qian Wang(University of South Carolina), David A. Tirrell(California Institute of Technology)
Cited by 302
Abstract
Playing tag: Noncanonical amino acid tagging enables the selective fluorescent visualization of newly synthesized proteins in mammalian cells (see the picture). Susceptibility to tagging is determined by the spatial and temporal character of the protein synthesis, thus providing a complement to methods which identify relevant members of the proteome.
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