Development of a Dinoflagellate-Oriented PCR Primer Set Leads to Detection of Picoplanktonic Dinoflagellates from Long Island Sound
Senjie Lin(University of Connecticut), Huan Zhang(University of Connecticut), Yubo Hou(University of Connecticut), Lilibeth Miranda(University of Connecticut), Debashish Bhattacharya(University of Iowa)
Cited by 68Open Access
Abstract
We developed dinoflagellate-specific 18S rRNA gene primers. PCR amplification using these oligonucleotides for a picoplanktonic DNA sample from Long Island Sound yielded 24 clones, and all but one of these clones were dinoflagellates primarily belonging to undescribed and Amoebophrya-like lineages. These results highlight the need for a systematic investigation of picodinoflagellate diversity in both coastal and oceanic ecosystems.
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